Frequently Asked Questions

What are the mean and geometric mean?

Why do contour plot quadrants not match histogram markers?

How many cells can you sort through in an hour?

I know I have to provide the collection media for the cells to be sorted in, but do I need to do or add anything special to it?

What kind of flow cytometry equipment do you have?

What dyes can your instruments read?


What are the mean and geometric mean? (Back to top)

Mean is the average channel number or linear value of the events within a marker set. Take the sum of all the values and divide by the number of values.

Geometric Mean is the average of the logs of the channel numbers or linear values of the events within a marker set, expressed as the anti-log. This is typically used when taking the log data versus linear data.

Why do contour plot quadrants not match histogram markers? (Back to top)

The reason the contour plot quadrants do not match histogram markers is because the contour plot uses Side Scatter as the parameter for the Y-axis, whereas the histogram only uses Counts (number of events) as the parameter for the Y-axis.

How many cells can you sort through in an hour? (Back to top)

On average, we can sort between 4-7 million cells per hour, depending on the concentration of the sample. We suggest preparing samples at a concentration of 5 million per milliliter with a maximum of 20 million per sample.

I know I have to provide the collection media for the cells to be sorted in, but do I need to do or add anything special to it? (Back to top)

We suggest preparing the collection media for the sorted cells to contain all necessary components for maximum growth of the cells. Depending on the type of cells, this may include, but is not limited to, adding extra serum and antibiotic. After the cells have begun to show growth after the sort, they may be re-cultured into their normal medium.

What kind of flow cytometry equipment do you have? (Back to top)

The equipment currently being used at Cytometry Research to provide state-of-the-art flow cytometry services is described here.

What dyes can your instruments read? (Back to top)

Our instruments can read the following dyes: FITC, 7AAD, Cychrome, PE, PI, H2DCFDA (DCF), PE-CY5, CY3, GFP, PE-CY7, DHE, Alexa488, and PerCP. Choose up to three dyes on the appointment form.

 

 

 

 

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